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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 10, No. 2, 2011, pp. 153-159
Bioline Code: pr11022
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 10, No. 2, 2011, pp. 153-159

 en Absence of Mutagenicity in Three Nigerian Medicinal Plants - Bidens pilosa check for this species in other resources , Cleistopholis paterns check for this species in other resources and Tetrapleura tetraptera check for this species in other resources
Hong, Chang-Eui; Ji, Seang-Taek & Lyu, Su-Yun

Abstract

Purpose: Bidens pilosa check for this species in other resources , Cleistopholis paterns check for this species in other resources , and Tetrapleura tetraptera check for this species in other resources are plants that are used traditionally for cancer treatment, as well as anti-bacterial, and anti-inflammatory agents in Africa. We used the Ames test to evaluate the bacterial reverse mutation effects in these plants.
Methods: In the in vitro Ames test, Salmonella typhimurium check for this species in other resources strains TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli check for this species in other resources WP2uvrA were used with or without metabolic activation by S9 mix. The highest concentration of B. pilosa, C. paterns, and T. tetraptera for the Ames test was established at 5000 µg/plate.
Results: B. pilosa, C. paterns, and T. tetraptera did not cause any increase in the number of his+ revertant colonies compared to the negative control values obtained from S. typhimurium and S. typhimurium WP2uvrA strains, with or without the addition of S9 mixtures. The results indicated that these plants are non-mutagenic to all the five S. typhimurium and S. typhimurium test strains in the presence and absence of metabolic activation.
Conclusion: B. pilosa, C. paterns, and T. tetraptera showed negative results in the bacterial reverse mutation test, suggesting that it is potentially safe to use them as medicinal plant supplements even at high doses.

Keywords
Bidens pilosa; Cleistopholis paterns; Tetrapleura tetraptera; Ames test

 
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