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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 10, No. 6, 2011, pp. 705-711
Bioline Code: pr11084
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 10, No. 6, 2011, pp. 705-711

 en A Protease Isolated from the Latex of Plumeria rubra check for this species in other resources Linn (Apocynaceae) 1: Purification and Characterization
Chanda, Indranil; Basu, Sanat Kumar; Dutta, Sadhan Kumar & Das, Smriti Rekha Chanda

Abstract

Purpose: To isolate, purify and characterize protease from the latex of the plant.
Methods: Protease was isolated from the latex of Plumeria rubra Linn using acetone precipitation method and purified by a sequence of DEAE cellulose column chromatography, followed by two successive column purification in Sephadex G-50 and Sephadex G-200. The molecular weight of the purified protease was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE). The protease was given a trivial name, Plumerin-R.
Results: Plumerin-R showed a single protein band on SDS-PAGE and molecular weight was approximately 81.85 kDa. It remained active over a broad range of temperature but had optimum activity at 55 °C and pH 7.0 when casein was used as substrate. Activation of the protease by a thiol-activating agent indicated the presence of sulfhydryl as an essential group for its activity.
Conclusion: A protease from the latex of Plumeria rubra Linn was purified to homogeneity by a simple purification procedure and then characterized.

Keywords
Protease, Plumerin-R, Sulfhydryl, Purification; Characterization

 
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