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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 10, No. 6, 2011, pp. 785-791
Bioline Code: pr11094
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 10, No. 6, 2011, pp. 785-791

 en Antibacterial and Cytotoxic Activities of Acacia nilotica check for this species in other resources Lam (Mimosaceae) Methanol Extracts Against Extended Spectrum Beta-Lactamase Producing Escherichia coli check for this species in other resources and Klebsiella check for this species in other resources Species
Riaz, Saba; Faisal, Muhammad; Hasnain, Shahida & Khan, Naveed Ahmed

Abstract

Purpose: To investigate lysates from Acacia nilotica pods for their antimicrobial and cytotoxic activities against a variety of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as well as methicillin resistant Staphylococci aureus (MRSA).
Methods: ESBLs-producing Escherichia coli and Klebsiella spp were isolated from clinical and environmental specimens and incubated with Acacia nilotica pod lysates. The bactericidal activity and cytotoxic effects of the lysates were evaluated while fast protein liquid chromatography (size exclusion FPLC) was used to identify the various compounds showing bactericidal activity.
Results: The lysates showed remarkable bactericidal properties, killing almost 100 % of the bacteria they were tested against, including neuropathogenic Escherichia coli, MRSA, and Klebsiella spp. The bactericidal activity was heat-resistant and showed minimal cytotoxic effects on human brain microvascular endothelial cells. FPLC revealed eight peaks, with three of them representing compounds that had maximum bactericidal activity against all the tested isolates, but showed <30 % host cell cytotoxicity.
Conclusion: The lysate of Acacia nilotica pods is a potentially good candidate for the therapy of antibacterial-resistant bacteria, and would therefore require further studies.

Keywords
Acacia nilotica, ESBLs, MRSA, E. coli, Klebsiella, Antibacterial resistance, Cytotoxicity

 
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