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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 10, No. 6, 2011, pp. 809-815
Bioline Code: pr11097
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 10, No. 6, 2011, pp. 809-815

 en Simultaneous Spectrophotometric Determination of Valsartan and Ezetimibe in Pharmaceuticals
Ramachandran, Sridevi; Mandal, Badal Kumar & Navalgund, Sameer G

Abstract

Purpose: To develop a direct, simple and extraction-free spectrophotometric method for the simultaneous estimation of valsartan and ezetimibe in pharmaceuticals.
Methods: A spectrophotometric method for the determination of valsartan and ezetimibe was developed using acidic dyes, namely, bromophenol blue (BPB) and bromocresol green (BCG). The method was based on selective ion-pair formation between valsartan and the acidic dye. The yellow coloured ion-pair induces a bathochromic shift in the spectrum with maximum absorbance at 425 and 428 nm for BPB and BCG, respectively. The developed method was validated as per ICH guidelines.
Results:With BPB, the ion-pair formed obeyed Beer`s law in the ranges 5 - 40 and 1 - 50 μg/mL for valsartan and ezetimibe, respectively. The assay data for valsartan and ezetimibe were, 99.39 ± 0.53 and 98.17 ± 0.91 %, respectively, for the commercial formulation, and 99.41 ± 0.48 and 98.16± 0.89 %, respectively, for the developed formulation. The method was validated and the correlation coefficient for valsartan and ezetimibe were 0.995 and 0.999, respectively. Recovery was in the range 99.3 - 100.3 %.
Conclusion: The proposed method is reproducible, accurate, robust and suitable for the simultaneous quantitative analysis of the studied drugs in bulk and dosage formulation.

Keywords
Valsartan, Ezetimibe, Bromophenol blue, Bromocresol green, Spectrophotometric method

 
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