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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 1, 2012, pp. 43-50
Bioline Code: pr12006
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 1, 2012, pp. 43-50

 en Methanol Extract of Polyopes lancifolius check for this species in other resources Inhibits the Expression of Pro-inflammatory Mediators in LPSstimulated BV2 Microglia Cells via Downregulation of the NF-κB Pathway
Jayasooriya, RGPT; Kang, Chang-Hee; Park, Sung-Yong; Choi, Yung Hyun; Moon, Dong-Oh & Kim, Gi-Young

Abstract


Purpose: This study is aimed at identifying the anti-inflammatory mechanisms of a methanol extract of Polyopes lancifolius check for this species in other resources (MEPL) in lipopolysaccharide (LPS)-stimulated BV2 microglia cells.
Methods: The expression of mRNA and protein were investigated RT-PCR and western blot analyses in LPS-stimulated BV2 microglial cells. The level of nitric oxide (NO) production was analyzed using Griess reaction. The release of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) were determined using sandwich ELISA. NF-κB activation was detected using EMSA methods.
Results: MEPL significantly suppressed NO production in LPS-stimulated BV2 cells without any cytotoxicity. The results also indicate that MEPL decreased the production of PGE2 and TNF-α in LPSstimulated BV2 cells. Furthermore, pretreatment with MEPL resulted in a downregulation of LPSinduced mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and TNF-α. Investigation of the effect of MEPL on nuclear factor-κB (NF-κB) activity, which is a potential transcriptional factor for regulating inflammatory genes such as iNOS, COX-2 and TNF-α, showed that MEPL substantially inhibited the LPS-induced DNA-binding activity of NF-κB. MEPL also suppressed the LPS-induced degradation and phosphorylation of IκBα, and it consequently blocked p65 translocation from the cytosol to the nucleus.
Conclusion: These data show that MEPL may regulate LPS-induced NO, PGE2, and TNF-α production by suppressing NF-κB activity.

Keywords
Polyopes lancifolius, Nitric oxide, Prostaglandin E2, Tumor necrosis factor-α, Nuclear factor-κB

 
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