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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 1, 2012, pp. 84-90
Bioline Code: pr12011
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 1, 2012, pp. 84-90

 en Effect of β-Glucuronidase on Extraction Efficiency of Silymarin from Human Plasma Samples Using Validated HPLC-UV Analysis
Usman, Muhammad; Ahmad, Mahmood; Dayo, Abdullah; Madni, Asadullah; Ali, Liaqat; Yousuf, Muhammad; Khan, Mahtab Ahmad; Munir, Abubakar; Sohail, Muhammad & Mahmood, Arshad

Abstract

Purpose: To investigate the effect of β-glucuronidase on the extraction efficiency of silymarin (mainly as silybin) from spiked human plasma using a sensitive and reproducible high performance liquid chromatography (HPLC) method.
Methods: The importance of β-glucuronidase was evaluated by comparing the extraction efficiency of silymarin in β-glucuronidase-treated and untreated plasma samples. Isocratic HPLC with simple UV detection (288 nm) was applied to analyze the major silymarin components using Thermo-Electron C18 column (200 mm, 4.6 mm I.D., 5μm particle size). The mobile phase, consisting of methanol and 20 mM potassium dihydrogen phosphate buffer (50:50 v/v pH 2.8), was pumped at 1 ml/min.
Results: The mean extraction efficiency was 98.97 % (CV = 1.69 %) for treated and 40.88 % (CV = 2.77 %) for untreated plasma samples, compared with nominal concentrations.
Conclusion: The studied method showed 60 % reduced extraction efficiency of untreated samples compared to treated samples.

Keywords
Silymarin, Silybin, Extraction Efficiency, β-glucuronidase, HPLC

 
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