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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 1, 2012, pp. 117-124
Bioline Code: pr12015
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 1, 2012, pp. 117-124

 en Phenolic Content, and Antioxidant and Antimicrobial Activities of Crataegus Oxyacantha L (Rosaceae) Fruit Extract from Southeast Serbia
Kostic, Danijela A; Velickovic, Jasmina M; Mitic, Snezana S; Mitic, Milan N & Randelovic, Sasa S

Abstract

Purpose: The aim of this work was to determine the content of total phenols, total flavonoids, anthocyanins, as well as antioxidant and antimicrobial activities of hawthorn ( Crataegus oxyacantha check for this species in other resources L.) alcohol, hydroalcohol and aqueous extracts.
Methods: The content of total phenols, flavonoids and anthocyanins of the alcohol, hydroalcohol and aqueous extracts of hawthorn were determined using spectrophotometric methods. Antioxidant assay was based on the measurement of DPPH absorbance at 517 nm caused by the reaction of DPPH with the test sample. Antimicrobial activity was evaluated by measuring the zone of inhibition against selected test microorganisms: Bacillus subtilis check for this species in other resources , Staphylococcus aureus check for this species in other resources , Escherichia coli check for this species in other resources , Pseudomonas aeruginosa check for this species in other resources , and Salmonella abony check for this species in other resources while antifungal activity was tested against two organisms: Aspergillus niger check for this species in other resources and Candida albicans check for this species in other resources .
Results: The results of spectrophotometric investigations indicate that the content of total phenol compounds in the investigated extracts varied from 2.12 to 30.63 mg GAE g-1 of fresh hawthorn sample. The content of anthocyanins ranged from 0.3207 to 3.168 mg of cyanidin-3-O-glucoside g-1 of fresh hawthorn fruit. The fruit extracts showed high antioxidant activity with DPPH radical transformation value as high as 89.9 % in the methanol-water (50/50, v/v%)) extract. The ethanol extract exhibited antimicrobial activity against all test microorganisms except two, Bacillus subtilis and Staphylococcus aureus, and one species of fungi, Aspergillus niger.Flavonoid structure influenced the extract’s selectivity towards Gram-positive and Gram negative bacteria.
Conclusion: Extracts of the fruit of Crataegus oxyacantha L. can be used as natural antioxidant and antimicrobial preparations.

Keywords
Crataegus oxyacantha L.; Natural phenolic compounds; Antioxidant and antimicrobial activity, Southeast Serbia.

 
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