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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 2, 2012, pp. 201-207
Bioline Code: pr12025
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 2, 2012, pp. 201-207

 en Cytotoxic Activity of Hexane Extracts of Psidium Guajava check for this species in other resources L (Myrtaceae) and Cassia Alata check for this species in other resources L (Caesalpineaceae) in Kasumi-1 and OV2008 Cancer Cell Lines
Levy, Arkene S & Carley, Shanna-kay

Abstract


Purpose: The cytotoxic effects of hexane extracts of Cassia alata check for this species in other resources and Psidium guajava check for this species in other resources leaves were evaluated in OV2008 ovarian and Kasumi-1 leukemia cancer cell lines, respectively.
Methods: The cancer cells were exposed to various concentrations of either C. alata (100 – 180 µg/ml) or P. guajava (100 – 500 µg/ml) leaf extract for 24 h. Following treatment, the cells were evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the cytotoxic effect of the extracts. C. alata extract was also analyzed using high performance liquid chromatography (HPLC).
Results: C. alata and P. guajava extracts produced significant (p<0.05) cytotoxicity in OV2008 and Kasumi-1 cell lines, respectively. The IC50 values were 160 µg/ml for C. alata and 200 µg/ml for P guajava. Further, the cytotoxicity exhibited by C. alata might be attributable to the flavonoid, kaempferol, which was identified as a constituent of the extract.
Conclusion: The results suggest that further chemical analysis and mechanistic investigations should be conducted on P. guajava and C. alata extracts to validate their potential uses for anticancer therapy.

Keywords
P. guajava, C. alata, Cytotoxicity, Kasumi-1, Cancer cell, OV2008

 
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