The cytotoxic effects of hexane extracts of
leaves were evaluated in OV2008 ovarian and Kasumi-1 leukemia cancer cell lines, respectively.
The cancer cells were exposed to various concentrations of either C. alata
(100 – 180 µg/ml) or P. guajava
(100 – 500 µg/ml) leaf extract for 24 h. Following treatment, the cells were evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the cytotoxic effect of the extracts. C. alata
extract was also analyzed using high performance liquid chromatography (HPLC).
Results: C. alata
and P. guajava
extracts produced significant (p<0.05) cytotoxicity in OV2008 and Kasumi-1 cell lines, respectively. The IC50
values were 160 µg/ml for C. alata
and 200 µg/ml for P guajava
. Further, the cytotoxicity exhibited by C. alata
might be attributable to the flavonoid, kaempferol, which was identified as a constituent of the extract.
The results suggest that further chemical analysis and mechanistic investigations should be conducted on P. guajava
and C. alata
extracts to validate their potential uses for anticancer therapy.