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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 2, 2012, pp. 217-223
Bioline Code: pr12027
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 2, 2012, pp. 217-223

 en Acute and Subacute Toxic Study of Aqueous Leaf Extract of Combretum Molle check for this species in other resources
Yeo, Dodehe; Bouagnon, Rita; Djyh, Bernard Nazaire; Tuo, Chonta & N’guessan, Jean David

Abstract

Purpose: The purpose of the present study was to evaluate the acute and subacute toxicity of the aqueous leaf extract of Combretum molle check for this species in other resources .
Methods: The acute toxicity of the extract was evaluated in rats. The animals were orally administered with doses ranging from 2000 to 8000 mg/kg and observed continuously for the first 4 h, then hourly for the next 24 h, and finally, 6-hourly for 72 h. Control animals received orally normal saline. The rats were observed carefully for mortality, pain as well as respiratory movements. For subacute toxicity, 6 groups of 6 rats (3 male and 3 female) each received intraperitoneally, normal saline (control), 400, 600, 800, 1000 and 1200 mg/kg of the extract, respectively, thrice daily for 15 days. At the end of the treatment period, the animals were sacrificed and their organs (liver, heart and kidney) removed for macroscopic examination.
Results: For the acute toxicit test, no death and signs of poisoning were observed in the treated groups. In the subacute tstudy, LD50 in the rats after intraperitoneal administration was 700 mg/kg (456 - 896, 95 % confidence interval). The clinical signs of poisoning (motor difficulties, decreased respiratory rate, and tremor preceding death) were observed, suggesting overt toxicity throughout the neuromuscular system. However, histological examination of vital organs showed normal architecture suggesting no morphological abnormalities in the heart, kidney and liver.
Conclusion: The results show that the aqueous leaf extract of C. molle is moderately toxic when given intraperitoneally.

Keywords
Combretum molle, Acute/subacute toxicity, Histopathology, Rat.

 
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