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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 2, 2012, pp. 275-279
Bioline Code: pr12035
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 2, 2012, pp. 275-279

 en A Spectrophotometric Method for the Determination of Ramipril in Solid Dosage Forms
Afieroho, OE; Okorie, O & Okonkwo, TJN

Abstract

Purpose: To develop a simple and cost effective spectrophotometer method for the determination of ACE inhibitor ramipril in dosage forms.
Methods: UV spectrophotometry was used to develop and validate a simple method for the assay of ramipril in solid dosage form at λmax of 210 nm, as per International Conference on Harmonization (ICH) guidelines. Aqueous methanol (5 %) was used as the blank solvent. The method was validated for linearity, recovery, accuracy, precision, specificity in the presence of excipients, and also for inter-day stability under laboratory conditions.
Results: Validation results showed linearity in the range 1 – 38 µg/ml; recovery accuracy 101.55%; regression equation Y = 0.0256X + 0.0697, R2 of 0.9942; precision RSD <2.00 %; and negligible interference from common excipients and colorants. The method was accurate (95 % confidence limit) compared to standard liquid chromatography (LC) method, with comparable reproducibility when used to assay a commercial product (Ramitace®, 2 and 5 mg tablets).
Conclusion: The validated data were within allowable limits and therefore, the proposed method is recommended for routine quality control (QC) analysis

Keywords
Ramipril, Spectrophotmetric assay, Validation, Solid dosage forms

 
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