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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 11, No. 3, 2012, pp. 397-404
Bioline Code: pr12048
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 3, 2012, pp. 397-404

 en Inhibition of iNOS and DNA Oxidation by Methanol Extract of Schizonepeta tenuifolia check for this species in other resources
Tae, Kyoung-Seok & Kim, Sung-Jin

Abstract

Purpose: To investigate the antioxidant properties of the methanol extract of Schizonepeta tenuifolia check for this species in other resources as well as its effect on inducible nitric oxide synthase (iNOS) and cycleooxygenase-2 (COX-2) expression in lipopolysaccharides (LPS)-induced cell damage in macrophage cells.
Methods: The antioxidant activities of the plant extract were explored by measuring free radical scavenging activity, viz, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO), and deoxyribose oxidation levels. The mechanism of antioxidant action of the extract was determined by Western blot analysis for iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells.
Results: The extract contained antioxidant components, including phenolics, flavonoids and anthocyanin and exerted significant radical scavenging activity in a dose-dependent manner. It also produced deoxyribose oxidation and dramatic reducing power. Production of iNOS induced by LPS was significantly inhibited by the extract, suggesting that the extract inhibits nitric oxide (NO) production by suppressing iNOS expression. However, COX-2 induced by LPS was not significantly affected by the extract.
Conclusion: These results suggest that the methanol extract of S. tenuifolia exerts significant antioxidant activity via inhibition of free radicals, iNOS and DNA oxidation.

Keywords
Schizonepeta tenuifolia, NO, iNOS expression, COX-2, Deoxyribose oxidation, Antioxidant

 
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