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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 3, 2012, pp. 405-412
Bioline Code: pr12049
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 3, 2012, pp. 405-412

 en Methanol Extract of Polyopes lancifolius Suppresses Tumor Necrosis Factor-α-Induced Matrix Metalloproteinase-9 Expression in T24 Bladder Carcinoma Cells
Jayasooriya, R.G.P.T.; Nam, Taek-Jeong; Kim, Gi-Young & Choi, Yung Hyun

Abstract

Purpose: To investigate the effects of the methanol extract of Polyopes lancifolius check for this species in other resources (MEPL) on the expression of matrix metalloproteinase-9 (MMP-9) and invasion in T24 human bladder carcinoma cells.
Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analyses were performed to assess the expression of MMP-9 and its regulatory proteins. MMP-9 activity was evaluated using zymography while matrigel infiltration was performed to assess T24 bladder carcinoma invasion. Electrophoretic mobility assay was used to investigate the nuclear factor-κB (NF-κB) activity.
Results: The expression and activity of MMP-9 were significantly increased in response to TNF-α, but MEPL suppressed TNF-α-induced MMP-9 expression and activity. MEPL also inhibited TNF-α-induced MMP-9 expression at the transcriptional level by blocking the activation of the NF-κB signaling pathway. Furthermore, the extract suppressed TNF-α-induced phosphorylation of IκBα and consequently sustained cytosolic p65 and p50 expression. Matrigel invasion assay showed that MEPL significantly reduced TNF-α-induced invasion of T24 bladder carcinoma cells.
Conclusion: Collectively, these data indicate that MEPL regulates TNF-α-induced MMP-9 expression by suppressing NF-ΚΒ activity.

Keywords
Polyopes lancifolius, Matrix metalloproteinase-9, Invasion; Nuclear factor-κB

 
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