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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 5, 2012, pp. 729-737
Bioline Code: pr12086
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 5, 2012, pp. 729-737

 en Enhanced Recovery and Identification of a Tryptamine-Related Antibiotic Produced by Intrasporangium check for this species in other resources N8 from KwaZulu-Natal, South Africa
Okudoh, Vincent I & Wallis, Frederick M

Abstract

Purpose: To isolate and identify an antibiotic produced by a soil bacterium, Intrasporangium strain N8, with antibacterial activity against both Gram-positive and Gram-negative bacteria.
Methods: Fermentation followed by extraction using a three-solvent system (petroleum ether, acetone and ethyl acetate) and pH precipitation, successfully separated the antibiotic complex from the culture broth. Purification was carried out using flash column chromatography (FCC), thin-layer chromatography (TLC) and reverse phase high performance liquid chromatography (HPLC). The identities of the molecules were elucidated by gas chromatography-mass spectrometry (GC-MS) analysis.
Results: Three main components of the antibiotic were isolated and identified as 4-methyl-3-penten-2- one, 4-hydroxy-4-methyl-2-pentanone and N-acetyltryptamine. Bioassay results showed activity against both mammalian and plant pathogenic bacteria including Pseudomonas fluorescens check for this species in other resources , Xanthomonas campestris pv campestris check for this species in other resources , Escherichia coli check for this species in other resources and Serratia marcescens check for this species in other resources . Pseudomonas fluorescens (MIC = 0.0625 μg/ml) and Xanthomonas campestris pv campestris (MIC = 0.0026 μg/ml) represent the two plant pathogenic genera that are notoriously difficult to contain in the field.
Conclusions: Since the antibiotic isolated during this study showed activity against both mammalian and plant pathogenic bacteria, it is hoped that this work will encourage further investigation in this field. This antibiotic could become very useful as an agricultural bacteriocide against some resistant plant pathogens.

Keywords
Intrasporangium N8, Tryptamine, Antibiotic, Purification, Pathogenic bacteria.

 
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