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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 5, 2012, pp. 807-813
Bioline Code: pr12096
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 5, 2012, pp. 807-813

 en Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea check for this species in other resources (Polygalaceae)
Nwidu, Lucky Legbosi; Cilli, Eduardo Maffud & Vilegas, Wagner

Abstract

Purpose: To evaluate the amino acid, antioxidant and ionic profiles of Carpolobia lutea check for this species in other resources leaf (Polygalaceae) extract (CLL).
Methods: The powdered leaf was macerated and subjected to gradient solvent extraction with nhexane, chloroform, ethyl acetate and ethanol for 72 h to obtain their respective fractions. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2-Diphenyl-1-picrylhydrazyl DPPH while elemental and ionic analyses were carried out by atomic absorption spectrophotometry and potentiometric titration, respectively.
Results: MProline, alanine, serine, valine, glycine, glutamate and lysine were found in the ethanol fraction while lysine, phenyl alanine, glycine and serine were present in the ethyl acetate fraction but not in the non-polar fractions, n-hexane and chloroform. The ethyl acetate fraction contained more lysine, phenyl alanine, glycine and serine the other leaf fractions. Minimal radical scavenging activity of all the fractions was recorded. The most abundant cations in the extract were potassium and phosphorus (2.16 ± 0.05 and 1.90 ± 0.06 mg/g, respectively) while the most abundant anion was phosphate with a concentration of 23.23 ± 4.61 mg/g for the aqueous leaf fraction.
Conclusion: The study shows that CLL fractions contain variety of amino acids which could promote wound healing, as well as major and minor elemental ions which, as essential body electrolytes, are required for various metabolic processes in the body.

Keywords
Carpolobia leaf, Polygalaceae, Amino acid, Antioxidant, Ionic profile.

 
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