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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 6, 2012, pp. 887-891
Bioline Code: pr12104
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 6, 2012, pp. 887-891

 en A Novel Biological Synthesis of Gold Nanoparticle by Enterobacteriaceae check for this species in other resources Family
Honary, Soheyla; Gharaei-Fathabad, Eshrat; Paji, Zahra Khorshidi & Eslamifar, Masumeh

Abstract

Purpose: To demonstrate eco-friendly biosynthesis of gold nanoparticles by Enterobacteriaceae check for this species in other resources .
Methods: Pure colonies of nine different bacteria from the Enterobacteriaceae family were separated from water and cultured in Luria Bertani broth medium. Their respective supernatants were examined for ability to produce gold nanoparticles. In this step, 1 mM solution of Gold(III) chloride trihydrate H(AuCl4) added to reaction matrices (supernatant) separately. The reaction was performed in a dark environment at 37 °C. After 24 h, it was observed that the color of the solutions turned to dark purple from light yellow. The gold nanoparticles were characterized by UV-Visible spectroscopy, dynamic light scattering, scanning electron microscopy and Fourier transform infrared spectroscopy (FTIR) for yield, particle size, shape and presence of different functional groups, respectively. The nanoparticles were centrifuged and re-dispersed in double distilled water thrice to purify them for FTIR studies.
Results: The gold nanoparticles were fairly uniform in size, spherical in shape and with Z-average diameter ranging from 11.8 to 459 nm depending on the bacteria used. FTIR spectra revealed the presence of various functional groups in the gold nanoparticles which were also present in the bacterial extract.
Conclusion: The current approach suggests that rapid synthesis of nanoparticles would be feasible in developing a biological process for mass scale production of gold nanoparticles.

Keywords
Biosynthesis, Enterobacteriaceae, Gold nanoparticles

 
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