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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 11, No. 6, 2012, pp. 925-931
Bioline Code: pr12109
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 11, No. 6, 2012, pp. 925-931

 en Investigation of the Genetic Basis of Tetracycline Resistance in Staphylococcus aureus check for this species in other resources from Pakistan
Ullah, Farhat; Malik, Salman Akbar; Ahmed, Jawad; Ullah, Farman; Shah, Syed Majid; Ayaz, Muhammad; Hussain, Sajid & Khatoon, Lubna


Purpose: To determine the prevalence and genetic basis of tetracycline resistance in Staphylococcus aureus check for this species in other resources .
Methods: One hundred and thirty (130) clinical isolates of S. aureus were collected from Khyber Teaching Hospital, Peshawar, Pakistan. Susceptibility to antibiotics (doxycycline, tetracycline and minocycline) was determined by Kirby-Bauer disc diffusion method with minimum inhibitory concentration (MIC) evaluated on Muller-Hinton agar as described by the Clinical and Laboratory Standards Institute (CLSI). The tetracyclineresistant strains (TET-R) were screened by polymerase chain reaction (PCR) for the presence of four common tetracycline resistance determinants, viz, tet(K), tet(L), tet(M) and tet(O).
Results: Sixty (46.0 %) of these isolates were methicillin-resistant S. aureus (MRSA) while 70 (54.0 %) were methicillin-susceptible S. aureus (MSSA). Seventy four (56.9 %) strains were resistant to tetracycline (TET-R), 30 (23.1 %) to minocycline and 23 (17.7 %) to doxycycline. A majority of the MRSA were resistant to tetracyclines and all the MSSA were sensitive to doxycycline and minocycline. The tet(K) gene was found in 58 isolates and tet(L) in one isolate. No tet(M) and tet(O) were detected.
Conclusion: This study indicates that resistance to tetracyclines is mainly by efflux pumps mediated by tet(K) in S. aureus in northwestern Pakistan.

Staphylococcus aureus, Antimicrobial susceptibility, Antibiotic resistance, Tetracycline, Pakistan

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