This study aimed to evaluate the effects of topically applied water-in-oil (w/o) emulsion of
using standard R cutometer parameters.
A w/o emulsion of 1 % hydro-alcoholic extract of H. rhamnoides
(formulation) and placebo
control (base) were used in the study. Eleven healthy male volunteers with a mean age of 24.5 years
were selected after obtaining informed consent. The subjects were assigned to blindly use either the
formulation or the base for 7 consecutive weeks. The skin mechanical parameters determined with a
cutometer, were R0 (first maximum amplitude), R2 (gross-elasticity), R6 (viscoelasticity), R7 (biological
elasticity) and R8 (total recovery). In addition, the antioxidant activity of the formulation was evaluated
by 1, 1-diphenil-2-picrylhydrazyl (DPPH) method.
Topical application of 1 % organic extract emulsion of H. rhamnoides
improved most of the
biomechanical parameters evaluated when compared to the base group (p < 0.05). However, skin
extensibility and firmness of the active formulation- and base-treated groups (R0) were not different (p <
0.01). Of note, a significant correlation between the active formulation and the improvement of the skin
mechanical parameters was observed. The active formulation was found to be superior than the placebo
The topical antioxidant emulsion of H. rhamnoides
significantly improved skin
biomechanical parameters after 7 weeks of treatment. The data obtained suggest that H. rhamnoides
could be an alternative pharmacological tool for treating age-related loss of skin elasticity.