Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 12, No. 1, 2013, pp. 13-18
Bioline Code: pr13003
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 12, No. 1, 2013, pp. 13-18
© Copyright 2013 - Tropical Journal of Pharmaceutical Research
Formulation and Evaluation of Bupivacaine-Loaded Glutaraldehyde-Crosslinked High Molecular Weight Chitosan Microspheres|
Shivashankar, Murugesh & Mandal, Badal Kumar
Purpose: To develop a chitosan microsphere carrier system of bupivacaine for buccal administration.
Methods: Chitosan microspheres loaded with bupivacaine were prepared by emulsification technique
based on glutaraldehyde cross-linking and drug-loaded chitosan microsphere were coated with polyglycolic
acid (PGA) film The formulated microspheres were characterized by x-ray diffraction (XRD),
differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and in vitro release was
performed in pH 7.4 phosphate buffer both in the presence and in absence of lysozyme.
Results: Encapsulation yield was 83.1 %. SEM studies indicate that the microspheres were spherical
and had a relatively smooth surface. XRD and DSC data indicate that there was no interaction between
the drug and polymer. In vitro results show that in the presence of lysozyme, 39 % of the drug was
released from the microspheres after 4.5 days while maximum drug release (42.5 %) was achieved on
day 11. This compares with 31 % drug release on 4.5th day and 38 % on 11th day; maximum drug
release occurred on day 11 in the absence of lysozyme.
Conclusion: It is evident from this study that microspheres can potentially be used for controlled
release of of bupivacaine for the management of dental pain in the buccal cavity.
Cross-linked chitosan, Microsphere, In vitro release, Oral drug delivery, Bupivacaine, Local anesthesia.
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