Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 12, No. 1, 2013, pp. 33-38
Bioline Code: pr13006
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 12, No. 1, 2013, pp. 33-38
© Copyright 2013 - Tropical Journal of Pharmaceutical Research
In vitro and In vivo Characterisation of Piroxicam-Loaded Dika Wax Lipospheres|
Brown, Sinye A.; Chime, Salome A.; Attama, Anthony A.; Agu, Confidence I. & Onunkwo, Godswill C.
Purpose: To formulate piroxicam-loaded lipospheres and evaluate their in vitro and in vivo properties.
Method: Piroxicam-loaded lipospheres were prepared by hot homogenization technique using dika wax
and Phospholipon® 90G (1:1, 1:2 and 2:1) as the lipid matrix. Characterisation, based on particle size
and morphology, pH, drug content and encapsulation efficiency, were carried out on the lipospheres. In
vitro release was evaluated in simulated intestinal fluid (pH 7.5). Anti-inflammatory and ulcerogenic
properties of the piroxicam-loaded lipospheres were studied using healthy, adult Wistar rats.
Result: Photomicrographs revealed spherical particles in the range of 1.66 – 3.56 μm. The results also
indicated that lipospheres formulated with lipid matrix 1:1 and containing 0.25 % piroxicam had the
highest encapsulation efficiency of 84 %. In vitro release data showed that lipospheres formulated with
lipid matrix having higher concentration of dika wax exhibited the fastest drug release of drug with
maximum release time between 60 - 70 min. The lipospheres exhibited good anti-inflammatory
properties with 58.6 % oedema inhibition at 5 h. Piroxicam-loaded liposheres had an ulcer index of zero
while, the reference (plain piroxicam) had an ulcer index of 15.00 ± 1.23 (p < 0.05).
Conclusion: Piroxicam lipospheres formulated with a mixture of dika wax and phospholipid exhibited
good in vitro and in vivo properties.
Dika wax, Lipospheres, Piroxicam, Phospholipid, Ulcerogenicity, Anti-inflammatory
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