Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 12, No. 1, 2013, pp. 39-44
Bioline Code: pr13007
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 12, No. 1, 2013, pp. 39-44
© Copyright 2013 - Tropical Journal of Pharmaceutical Research
Comparison of Flow-Through Cell and Paddle Methods for Testing Vaginal Tablets Containing a Poorly Water-Soluble Drug|
Szymanska, Emilia & Winnicka, Katarzyna
Purpose: To evaluate the usefulness of the flow-through cell apparatus for testing commercial vaginal
tablets containing poorly water-soluble clotrimazole.
Methods: The effect of experimental conditions (type of dissolution medium, flow rate and positioning of
the tablet) on the dissolution profile of clotrimazole were examined and optimal parameters for
conducting the experiments were determined. The amount of drug released was analyzed by high
performance chromatography (HPLC) at 210 nm. The usefulness of the flow-through cell apparatus was
compared to FDA recommended paddle apparatus.
Results: Using acetate buffer pH 5.2 containing 1 % SDS, both methods gave different dissolution
profiles. The paddle apparatus tended to give faster rate of dissolution (approx. 88.5 % during the first
20 min of the experiment), which was probably caused by higher agitation and greater surface area of
the drug-dissolution medium in a vessel. In the flow-through cell method, total drug release was
definitely slower and was observed after 2 to 5 h; at a flow rate of 16 ml/min, more than 80 % of the
drug dissolved after 30 min of the test. It was noticed that raising the flow rate of the dissolution medium
caused significantly higher drug release.
Conclusion: The results demonstrate that the flow-through cell method is reproducible and can be
successfully used for evaluating in vitro dissolution of clotrimazole from non-modified release tablets.
The slower rate of dissolution obtained in the flow-through cell method would help to distinguish
between different formulations.
Dissolution test, Flow-through cell method, Paddle method, Clotrimazole
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