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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 1, 2013, pp. 51-56
Bioline Code: pr13009
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 1, 2013, pp. 51-56

 en Improvement in the Production of L-Lysine by Overexpression of Aspartokinase (ASK) in C. glutamicum check for this species in other resources ATCC 21799
Rastegari, Hilda; Chiani, Mohsen; Akbarzadeh, Azim; Cheraghi, Sara; Saffari, Zahra; Mehrabi, Mohammad Reza; Farhangi, Ali & Ghassemi, Soheil

Abstract

Purpose: To clone Corynebacterium glutamicum check for this species in other resources ATCC21799 aspartokinase gene (EC 2.7.2.4) using shuttle expression vector pEKEx2 in order to increase lysine production.
Methods:C. glutamicum DNA was extracted and used for amplification of aspartokinase gene (ask) by cloning into an E. coli check for this species in other resources /C. glutamicum shuttle expression vector, pEKEx2. Initially, the recombinant vector transformed into E. coli DH5α and then into C. glutamicum.
Results: Electrophoresis of recombinant protein by SDS-PAGE showed that the molecular weight of the recombinant protein was 42 KD. The induction of recombinant vector by IPTG had an inhibitory effect on cell growth due to over-expression of the cloned gene. The results of lysine assay by Chinard method showed that lysine production increased about two-fold, compared with the parent strain, as a result of increased copy numbers of lysC gene in recombinant strain.
Conclusion: A two-fold increase in lysine production was observed by cloning of the ASK gene in C. glutamicum rather than in E. coli, due to the presence of lysine exporter channel which facilitates lysine extraction.

Keywords
LysC gene, Corynebacterium glutamicum, L- lysine, Cloning, Aspartokinase, E. coli

 
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