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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 1, 2013, pp. 57-61
Bioline Code: pr13010
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 1, 2013, pp. 57-61

 en In vitro Anti-proliferative and Apoptotic Activities of Eurycoma longifolia check for this species in other resources Jack (Simaroubaceae) on HL-60 Cell Line
Al-Salahi, Omar Saeed Ali; Zaki, Abdel-Hamid; Chan, Kit-Lam; Shah, Amin Malik; Al-Hassan, Faisal; Abdullah, Wan Zaidah & Yusoff, Narazah Mohd

Abstract

Purpose: To investigate the anti-proliferative, apoptotic and differentiating activities of Eurycoma longifolia check for this species in other resources root extracts on HL-60 leukemic cells.
Methods: HL-60 cells were treated with different partially purified sub-fractions (F1 – F3) derived from the resin chromatography of the crude methanol root extract of E. longifolia roots, at different doses and time points. The anti-proliferative activity of E. longifolia was assessed via cell counting and trypan blue exclusion. Apoptosis was evaluated via Annexin-V FITC/IP and Hoechst staining. Flow cytometry and Wright staining were used to assess its differentiation activities.
Results: F1 showed unremarkable growth inhibition rate while F2 and F3 showed growth inhibitory effects with median inhibitory concentration (IC50) values of 15.2 and 28.6 μg/ml, respectively. Treatment with F2 and F3 (100 μg/ml) for 96 h increased cell death from 3.3 to 95.5 and 76.3 %, respectively. Treatment with F2 (50 μg/ml) induced apoptosis by 14, 19.5 and 25 % after 24, 48 and 72 h, respectively. No differentiation activity was observed, as indicated by absence of myeloid maturation and a non-significant CD14 positivity (p > 0.05).
Conclusion: E. longifolia extract (F2) showed promising anti-leukemic activity and can be a candidate for the development of a drug for the treatment of acute promyelocytic leukemia (APL).

Keywords
Acute promyelocytic leukemia (APL), HL-60 cells, Eurycoma longifolia, Apoptosis, Antiproliferation, Differentiation

 
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