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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 1, 2013, pp. 111-116
Bioline Code: pr13018
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 1, 2013, pp. 111-116

 en Phytochemical Screening and In vitro Evaluation of Pharmacological Activities of Aphanamixis polystachya check for this species in other resources (Wall) Parker Fruit Extracts
Apu, Apurba Sarker; Chowdhury, Fariha Akhter; Khatun, Farjana; Jamaluddin, A.T.M.; Pathan, Atiqul Haque & Pal, Arindom

Abstract

Purpose: To investigate the crude n-hexane, ethyl acetate and methanol extracts of Aphanamixis polystachya check for this species in other resources fruit for their cytotoxic, antimicrobial, antioxidant and thrombolytic activities.
Methods: The fruit extracts were screened for major phytochemical compounds using in vitro established procedures. Antimicrobial and cytotoxic studies of the fruit extracts were conducted using disc diffusion and brine shrimp lethality bioassay methods, respectively, while an in vitro thrombolytic model was used to assess the clot lysis effect of the extracts with streptokinase as positive control. Antioxidant activity was evaluated by free radical scavenging activity using 2, 2-diphenyl-1- picrylhydrazyl (DPPH) and nitric oxide assay as well as total phenolic content.
Results: The fruit extracts were a rich source of phytochemicals and among the extracts n-hexane extract showed highest antimicrobial activity against Shigella dysenteriae check for this species in other resources (zone of inhibition: 9.7±0.2 mm) and Candida albicans check for this species in other resources (zone of inhibition: 8.8±0.3 mm) at a concentration of 1000μg/disc, whereas at the same concentration methanol extract showed highest zone of inhibition, 10.1±0.4mm, against Staphylococcus aureus. Compared to potassium permanganate with a median lethal concentration (LC50) of 13.23 μg/ml in the brine shrimp lethality assay, the LC50 of n-hexane, ethyl acetate and methanol extracts were 15.77, 17.51 and 141.37 μg/ml, respectively. All the extracts showed significant clot lysis activity (p < 0.001) with reference to negative control and % clot lysis of the extracts were approximately 13. Notable antioxidant activity of the methanol extract was observed unlike the other extracts.
Conclusion: The results of the study demonstrated the potential cytotoxic, thrombolytic and antioxidant activities of the fruit extracts of A. polystachya and therefore further studies on the isolation and identification of active principles are required.

Keywords
Aphanamixis polystachya, Antimicrobial, Antioxidant, Cytotoxic, Thrombolytic, Phytochemical screening

 
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