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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 3, 2013, pp. 351-356
Bioline Code: pr13054
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 3, 2013, pp. 351-356

 en Suaeda japonica check for this species in other resources Makino Attenuates Lipopolysaccharide-Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling
Kang, Hyun; Koppula, Sushruta; Kim, Hoi- Ki & Park, Tae-Kyu

Abstract

Purpose: To evaluate in vitro anti-oxidant and anti-neuro-inflammatory activities of Suaeda check for this species in other resources japonica extract (SJE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.
Methods: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay was used to study the antioxidant effects. 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay was used to study cell viabilities. LPS-stimulated BV- microglial cells were used to study the gene expression and production of inflammatory mediators determined by Western blot analysis.
Results: SJE significantly inhibited the DPPH generated free radicals, and suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) in a concentration-dependent manner. It decreased LPS-induced expression of some inflammatory mediators and pro-inflammatory cytokines (cyclooxygenase-2 and interleukin (IL)-6). This suppression of inflammatory mediators was nuclear factor kappa-B (NF-κB)-dependent.
Conclusion: Our findings imply that SJE may be a potential therapeutic agent in regulating microglia-mediated neuroinflammatory responses observed in several neurodegenerative diseases.

Keywords
Suaeda japonica; Antioxidant activity; Anti-inflammatory activity; Microglial Cells; IL-6; iNOS

 
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