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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 3, 2013, pp. 369-375
Bioline Code: pr13057
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 3, 2013, pp. 369-375

 en Inhibition of Inducible Nitric Oxide Synthase, Cycleooxygenase-2 and Lipid Peroxidation by Methanol Extract of Pericarpium Zanthoxyli check for this species in other resources
Chung, Jin-Won; Oh, Jin Uk; Lee, Sehyung & Kim, Sung-Jin

Abstract

Purpose: To explore the antioxidant properties of the methanol extract of Pericarpium Zanthoxyli check for this species in other resources and its effect on inducible nitric oxide synthase (iNOS), cycleooxygenase-2 (COX-2) and lipopolysaccharides (LPS)-induced cell damage in macrophage cells.
Methods: Anti-oxidant activities were tested by measuring free radical scavenging activity (DPPH, NO) and lipid peroxidation levels. The mechanism of anti-oxidant action of Pericarpium Zanthoxyli extract was determined by Western blot analysis for iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells.
Results: Pericarpium Zanthoxyli extract contained anti-oxidant components including phenolics (2.456 mg/g), flavonoids (0.127 mg/g) and anthocyanins (20.34 mg/g). The extract exerted significant radical scavenging activity in a dose-dependent manner. It also inhibited lipid peroxidation and exerted dramatic reducing power (28.9-fold compared with control at a concentration of 1 mg/ml). Production of iNOS induced by LPS was significantly (p < 0.05) inhibited by the extract, suggesting that the extract inhibits nitric oxide (NO) production by suppressing iNOS expression. Strikingly, COX-2 induced by LPS was also significantly (p < 0.05) inhibited by the extract.
Conclusion: These results suggest that the methanol extract of Pericarpium Zanthoxyli exerts significant anti-oxidant activity via inhibiting free radicals, iNOS and lipid peroxidation as well as by inhibition of COX-2 enzyme.

Keywords
Pericarpium Zanthoxyli; Nitric oxide; COX-2; iNOS; Lipid peroxidation; Antioxidant

 
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