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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 3, 2013, pp. 377-383
Bioline Code: pr13058
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 3, 2013, pp. 377-383

 en Antioxidant Activity and Cytotoxicity of the Leaf and Bark Extracts of Tarchonanthus camphorates check for this species in other resources
Nanyonga, Sarah K; Opoku, Andy R; Lewu, Francis B; Oyedeji, Opeoluwa O; Singh, Moganavelli & Oyedeji, Adebola O

Abstract

Purpose: To investigate the antioxidant potential and cytotoxicity of the leaf and bark extracts of Tarchonanthus camphorates check for this species in other resources .
Methods: The antioxidant activity of the aqueous leaf extract (Aq LF), methanol leaf extract (MET LF), dichloromethane leaf extract (DCM LF), methanol bark extract (MET BK), dichloromethane bark extract (DCM BK), and ethyl acetate bark extract (Et Ac BK) were examined by 1,1-Diphenyl-2-picryl-hydrazil (DPPH), 2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS.+), nitric oxide radical scavenging, reducing power and iron chelating activity methods. Brine shrimp lethality and MTT cytotoxicity tests were used to investigate the cytotoxicity of the extracts.
Results: The Aq LF, DCM LF, MET LF and MET BK showed good DPPH, ABTS radical scavenging and total reducing power activities. Total reducing power was high and in the rank order of DCM LF > Aq LF > MET LF > MET BK. All the extracts, however, showed weak nitric oxide scavenging activity as well as weak iron chelating ability. Flavonoids, phenols, tannins and saponins were present in some of the extracts, but alkaloids, terpenoids, cardenolides and cardiac glycosides were absent in all the extracts. All the extracts did not show significant cytotoxic properties (p ≥ 0.05) with 50% inhibitory concentration IC50 values > 30 μg/ml in both cytotoxicity assays.
Conclusion: The antioxidant activity and low cytotoxicity of Tarchonanthus camphoratus probably justify its use in folk medicine.

Keywords
Antioxidant activity; Cytotoxicity; Tarchonanthus camphoratus

 
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