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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 3, 2013, pp. 419-423
Bioline Code: pr13063
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 3, 2013, pp. 419-423

 en Ultrastructural Study of Elaeis guineensis check for this species in other resources (Oil Palm) Leaf and Antimicrobial Activity of its Methanol Extract against Staphylococcus Aureus check for this species in other resources
Rajoo, Amala; Sasidharan, Sreenivasan; Jothy, Subramanion L; Ramanathan, Surash & Mansor, Sharif M

Abstract

Purpose: To evaluate the antimicrobial activity of the methanol extract of Elaeis guineensis check for this species in other resources leaf against Staphylococcus Aureus check for this species in other resources and to determine the effect of extract treatment on the microstructure of the microbe
Methods: The antimicrobial activity of the methanol leaf extract of the plant against S. aureus was examined using disc diffusion and broth dilution methods. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were carried out to determine the major alterations in the microstructure of S. aureus after treatment with the extract.
Results: The extract showed a good antimicrobial activity against S. aureus with a minimum inhibition concentration (MIC) of 6.25 mg/mL and for Chloramphenicol was 30.00 µg/mL. The main changes observed under SEM and TEM were structural disorganization of the cell membrane which occurred after 12 h and total collapse of the cell 36 h after exposure to the extract.
Conclusion: We concluded that the methanolic extract of E. guineensis check for this species in other resources leaf exhibited good antimicrobial activity against S. aureus and this is supported by SEM and TEM.

Keywords
Antimicrobial activity; Elaeis guineensis; Staphylococcus aureus; Scanning electron microscopy; Transmission electron microscopy

 
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