Purpose: To investigate the inhibitory effect of polysaccharides from
Scutellaria barbata
(PSB) on invasion and metastasis of lung cancer, and study the possible mechanism.
Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and anti-metastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the expression of C-MET and E-CAD.
Results: The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p < 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p < 0.05). The expression of C-MET and E-CAD in 95-D cells treated with PSB were significantly down-regulated and up-regulated, respectivelt (p < 0.05).
Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD.
