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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 4, 2013, pp. 517-522
Bioline Code: pr13078
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 4, 2013, pp. 517-522

 en Inhibitory Effect of Polysaccharides from Scutellaria barbata check for this species in other resources D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD Expressions
Yang, Xiao-Kun; Yang, Ya-Dong; Tang, Shu-Qian; Xu, Ling; Yang, Gui-Hong; Xu, Qiao-Yu; Tang, Hui & Wu, Jin-Jin

Abstract

Purpose: To investigate the inhibitory effect of polysaccharides from Scutellaria barbata check for this species in other resources (PSB) on invasion and metastasis of lung cancer, and study the possible mechanism.
Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and anti-metastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the expression of C-MET and E-CAD.
Results: The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p < 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p < 0.05). The expression of C-MET and E-CAD in 95-D cells treated with PSB were significantly down-regulated and up-regulated, respectivelt (p < 0.05).
Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD.

Keywords
Polysaccharide; Scutellaria barbata; 95-D cell lines; Invasion; Metastasis

 
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