To isolate and identify the cytotoxicity of the constituents of Sarcopyramis bodinieri
Methods: S. bodinieri
var. delicate was extracted with hydrochloric acid-methanol and fractionated with
ethyl acetate further. The chemical constituents of the ethyl acetate fraction were purified by a
combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The structure
was characterized by 1
H-Nuclear Magnetic Resonance (NMR) and electrospray ionization tandem mass
spectrometry (ESI-MS). Apoptosis was evaluated by fluorescence staining and Western blot analysis
using 4,6-diamidino-2-phenylindole (DAPI) staining and poly (ADP-ribose) polymerase (PARP) SDSPAGE
tests in HepG2 liver cancer cells.
One flavonoid with high purity was purified by the combination of D101 macroporous resin and
Sephadex LH-20 column chromatography. The flavonoid compound was identified as quercetin by 1
and ESI-MS analyses. DAPI staining and PARP SDS-PAGE tests showed 60 μM quercetin could
induce potential apoptotic activity in HepG2 liver cancer cells.
Quercetin was the major cytotoxicity constituent in S. bodinieri