To evaluate the anticancer activity of an enzyme, methioninase, obtained from a soil isolate, Serratia marcescens
, against Dalton’s lymphoma ascitic (DLA) and Ehrlich ascitic carcinoma (EAC) in Swiss albino mice.
Cancer was induced using DLA and EAC cells in Swiss albino mice by intraperitoneal injection. The mice were then treated with 10 and 20 mg/kg body weight of methioninase; 5-fluorouracil (5-FU) was used as standard.
The results showed good improvement in haematological parameters, improvement serum enzymes and lipid profile, as well as increase in life span by up to 77 %, increase in body weight (p < 0.01), decrease in cancer cell count from (2.70 ± 0.40) × 106
to (1.86 ± 0.30) × 106
cells/ml compared to (2.70 ± 0.40) × 106
to (1.42 ± 0.25) x 106
cells/ml for the group treated with 5-FU. Solid tumor volume decreased from 5.82 ± 0.15 to 4.10 ± 0.11 ml as as against a decrease from 5.82 ± 0.40 to 3.56 ± 0.28 for the group treated with 5-FU.
The isolated enzyme, methioninase, has a good anti-cancer activity and needs to be further be investigated for this activity.