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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 5, 2013, pp. 719-725
Bioline Code: pr13088
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 5, 2013, pp. 719-725

 en In vitro Studies on the Inhibition of α-Amylase and α-Glucosidase by Leaf Extracts of Picralima nitida check for this species in other resources (Stapf)
Kazeem, Mutiu I; Ogunbiyi, Jesuyon V & Ashafa, Anofi OT

Abstract

Purpose: To investigate the anti-diabetic potential of Picralima nitida check for this species in other resources leaf extracts in vitro.
Methods: The current study evaluated the anti-diabetic potential of Picralima nitida leaf via in vitro inhibition of α-amylase and α-glucosidase using the acetone, water and ethanol extracts. Preliminary phytochemical analysis was performed on the acetone, aqueous and ethanol extracts of Picralima nitida leaf. The α-amylase inhibitory potentials of the extracts were investigated by reacting different concentrations of the extracts with α-amylase and starch solution while α-glucosidase inhibition was determined by pre-incubating α-glucosidase with different concentrations of the extracts followed by the addition of p-nitrophenylglucopyranoside (pNPG). The mode(s) of inhibition of both enzymes was determined using Lineweaver-Burke plot.
Results: The acetone extract of Picralima nitida displayed the most effective inhibition of both α-amylase and α-glucosidase activities with half-maximal inhibitory concentration (IC50) of 6.50 and 3.00 mg/ml, respectively. The results also showed that the extracts contain both non-competitive and competitive inhibitors of α-amylase and α-glucosidase respectively. The results of phytochemical analysis of the extract revealed the presence of saponins, flavonoids, tannins, terpenoids and reducing sugars.
Conclusion: The observed inhibitions of α-amylase and α-glucosidase suggest that the leaf extracts of P.nitida may be useful in the management of diabetes mellitus. This buttresses the traditional use of P.nitida for the management of sugar-related disorders in some parts of Nigeria.

Keywords
Picralima nitida; Diabetes; α-Amylase; α-Glucosidase; Hyperglycemia

 
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