search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 5, 2013, pp. 727-733
Bioline Code: pr13089
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 5, 2013, pp. 727-733

 en Dual Regulating Effect of Shaoyao-Gangcao-Tang on COX-2 Expression in Acute and Resolution Phases of Carrageenin-Induced Pleurisy in Rats
Chen, Gang; Tan, Ming-Liang; Gao, Xue & Jia, Ping

Abstract

Purpose: To investigate the effects and potential mechanisms of Shaoyao-Gangcao-Tang (SGT) on acute and resolution phases of carrageenin-induced pleurisy in rats.
Methods: To determine the effects of SGT at 2 h, Sprague-Dawley rats received injection of 0.2 ml of 1 % λ-carrageenin into the pleural cavity after treatment with 4.0, 13.3 and 40.0 g/kg SGT for three days. At 2 h after pleurisy induction, exudate volume, total cell number, prostaglandin E2 (PGE2) production and cyclooxygenase-2 (COX-2) protein expression were measured. To determine the effects at 48 h, the rats were treated with SGT at 24, 36 and 46 h after injection of λ-carrageenin into the pleural cavity, and the exudate volume, total cell number, 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2) production and COX-2 protein expression were evaluated.
Results: At 2 h after pleurisy induction, 13.3 and 40.0 g/kg SGT significantly decreased exudate volume by 34 (p < 0.05) and b 4 0% (p < 0.01), total cell number by 27 (p < 0.05) and 41 % (p < 0.01), PGE2 production by 17 (p < 0.05) and 35 % (p < 0.01), as well as COX-2 protein expression by 21 (p < 0.01) and 43 % (p < 0.01) compared with control group treated with saline. At 48 h after pleurisy induction, 13.3 and 40 g/kg SGT also significantly decreased exudate volume by 36 (p < 0.05) and 55 % (p < 0.01), as well as total cell number by 31 (p < 0.05) and 43 % (p < 0.01), but markedly increased 15d-PGJ2 production by 26 (p < 0.05) and 51 % (p < 0.01), as well as COX-2 protein expression by 50 (p < 0.01) and 100 % (p < 0.01) compared with control group.
Conclusion: The findings suggest that SGT has dual regulating effect in acute and resolution phases of inflammation, involving inhibiting acute inflammation through down-regulation of pro-inflammatory mediators, and promoting inflammatory resolution through up-regulation of pro-resolution mediators.

Keywords
Shaoyao-Gangcao-Tang; Cyclooxygenase-2; Prostaglandin E2; 15-Deoxy-Δ12; 14-PGJ2; Inflammation

 
© Copyright 2013 - Tropical Journal of Pharmaceutical Research
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil