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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 5, 2013, pp. 747-753
Bioline Code: pr13092
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 5, 2013, pp. 747-753

 en Anti-Proliferative, Antioxidant and Iron-Chelating Properties of the Tropical Highland Fern, Phymatopteris triloba check for this species in other resources (Houtt) Pichi Serm (Family Polypodiaceae)
Chai, Tsun-Thai; Quah, Yixian; Ooh, Keng-Fei; Ismail, Nor Ismaliza Mohd; Ang, Yee-Von; Elamparuthi, Sanmugapriya; Yeoh, Loo-Yew; Ong, Hean-Chooi & Wong, Fai-Chu


Purpose: To determine the phenolic constituents as well as anti-proliferative, antioxidant and iron-chelating activities of the leaf and rhizome extracts of Phymatopteris triloba check for this species in other resources .
Methods: Concentrations of selected hydroxybenzoic acids, hydroxycinnamic acids and flavonoids in aqueous extracts were quantified using high performance liquid chromatography (HPLC). Anti-proliferative activity was assessed on human cervix cancer cell line (HeLa) and human chronic myelogenous leukemia cell line (K562). Superoxide and nitric oxide scavenging activities as well as iron-chelating activity were determined colorimetrically.
Results: Protocatechuic acid content of the rhizome extract (154.7 g/g dry matter (DM)) was 1.9-fold higher than in the leaf extract. p-Hydroxybenzoic acid (34.6 g/g DM) and gallic acid (18.9 g/g DM), were only detected in the rhizome extract. Sinapic acid (6.6 g/g DM) was detected in the leaf extract only. Myricetin content of leaf extract (98.5 g/g DM) was 3.7-fold higher compared with the rhizome extract. At 500 g DM/ml, both extracts produced about 40 and 30 % anti-proliferative activity on HeLa cells and K562 cells, respectively. Both extracts had moderate nitric oxide-scavenging and iron-chelating activities. The leaf extract half-maximal effective concentration (EC50) value of 0.85 mg/ml (scavenging of superoxide radicals) was higher than that of ascorbic acid.
Conclusion: P. triloba is a potential source of anti-proliferative, antioxidant and iron-chelating agents. Its bioactivities may be attributed to the presence of phenolic constituents.

Bioactivity; Fern; Phenolics; Anti-proliferative; Anti-oxidant; Iron-chelating; Phymatopteris triloba

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