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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 12, No. 5, 2013, pp. 813-819
Bioline Code: pr13102
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 5, 2013, pp. 813-819

 en Antioxidant and Antimicrobial Activity of Polyphenol Extracts from Wild Berry Fruits Grown in Southeast Serbia
Radovanović, Blaga C; Anđelković, Ana S Milenković; Radovanović, Aleksandra B & Anđelković, Marko Z

Abstract

Purpose: To assess the antioxidant and antimicrobial activities of polyphenolic extracts of three wild red wild berry fruit species from Southeast Serbia, viz, European cornel ( Cornus mas check for this species in other resources ), blackthorn ( Prunus spinosa check for this species in other resources L.) and wild blackberry ( Rubus fruticosus check for this species in other resources ).
Methods: Polyphenol content was determined using spectrophotometric and High performance liquid chromatography (HPLC) methods. Antioxidant activity was estimated by 2,2`- diphenyl - 1 - picrylhydrazyl (DPPH) test while reliminary antimicrobial tests were carried out by disc diffusion method in which antibacterial activity was evaluated by measuring the zone of inhibition against test bacterial strains. Broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
Results: The highest amount of total phenols was found in European cornel (8625.89 mg kg-1FW). Galic acid, caffeic acid, p-coumaric acid, ferulic acid, (+)-catechin, procyanidin B2, (-)-epicatechin, quercetin, rutin and quercetin-3-glucoside were the main polyphenols in the fruit extracts. All extracts showed high scavenging effect on DPPH radical with IC50 values ranging from 22.19 to 31.18 ml g-1, as well as high antimicrobial activity on almost all the tested bacterial strains.
Conclusion: Extracts of wild berry fruits may be suitable for the preparation of medicinal and nutritional products.

Keywords
European cornel; Blackthorn; Wild blackberry; Polyphenols; Antioxidant; Antimicrobial

 
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