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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 12, No. 6, 2013, pp. 897-903
Bioline Code: pr13113
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 6, 2013, pp. 897-903

 en Salicornia bigelovii check for this species in other resources Torr Attenuates Neuro-Inflammatory Responses in Lipopolysaccharide-Induced BV-2 Microglia by Regulation of NF-kappa B Signaling
Kang, Hyun; Koppula, Sushruta & Park, Tae-Kyu


Purpose: To investigate the anti-oxidant and anti-neuroinflammatory effects of Salicornia bigelovii check for this species in other resources extract (SBE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.
Methods: Anti-oxidant activity was measured using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay. BV- microglial cells were stimulated with LPS to study the protein expression and production of inflammatory mediators, determined by Western blot analysis.
Results: SBE significantly inhibited the DPPH-generated free radicals showing maximum inhibition at 40 µg/mL (p < 0.001). SBE alone did not exhibit any signs of cytotoxicity to BV-2 cells up to 200 µg/mL concentration. The LPS-induced increase in the production of nitric oxide was concentration-dependently suppressed by SBE (p < 0.05 for 10 µg/mL, p < 0.01 at 20 µg/mL and p < 0.001 at 40 µg/mL, respectively). SBE also inhibited the LPS-induced increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. Further, the production of proinflammatory cytokines such as tumor necrosis factor-α and interleukin-6 by LPS-stimulation in BV-2 cells was inhibited by SBE pretreatment. Mechanistic study revealed that SBE acts by regulation of nuclear factor kappa-B signaling pathway in LPS-stimulated BV-2 microglial cells.
Conclusion: This study revealed for the first time that SBE possesses anti-oxidant and anti-neuroinflammatory effects and can be developed as a potential therapeutic target in ameliorating microglia-mediated neuroinflammation.

Salicornia bigelovii; Anti-oxidant; lipopolysaccharide; Neuroinflammation; Microglia; Cyclooxygenase; iNOS; NF-κB

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