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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 12, No. 6, 2013, pp. 913-918
Bioline Code: pr13115
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 6, 2013, pp. 913-918

 en Anti-inflammatory Effects of Magnolia sieboldii check for this species in other resources Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages
Oyungerel, Baatartsogt; Lim, Heekyong; Lee, Chi-Ho; Choi, Eun-Hee; Li, Guan-Hao & Choi, Kang-Duk


Purpose: To investigate the effect of Magnolia sieboldii check for this species in other resources extract (MSE) on the production of pro-inflammatory cytokines by macrophage.
Methods: The whole plant of M. sieboldii was extracted with methanol at room temperature. The in vitro anti-inflammatory activity of MSE was investigated on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LPS-induced nitric oxide(NO) production was determined by Griess method. Production of pro-inflammatory cytokines including Interleukin-1 beta (IL-1 β), Interleukin-6 (IL-6), tumor necrosis factor- alpha (TNF-α) and cyclooxygenase-2 (COX-2) was examined using reverse trancriptase - polymerase chain reaction (RT-PCR) and Western blot analysis.
Results: Under in vitro conditions, MSE in doses ranging from 25 - 100 μg/mL significantly inhibited lipopolysaccharide (0.5 μg/mL)-induced nitric oxide production (p < 0.001), and the production of pro-inflammatory mediators (p < 0.05).
Conclusions: The anti-inflammatory effect of MSE on pro-inflammatory cytokines seems to ameliorate inflammatory symptoms via immune regulation.

Anti-inflammatory; Nitric oxide; Cytokines; Immune regulation; Macrophage; Lipopolysaccharide; Polymyxin B; Magnolia sieboldii

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