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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 12, No. 6, 2013, pp. 929-933
Bioline Code: pr13117
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 6, 2013, pp. 929-933

 en Cytotoxicity of Essential Oil of Chenopodium ambrosioides check for this species in other resources L against Human Breast Cancer MCF-7 Cells
Jia-liang, Wu; Dan-wei, Ma; Ya-nan, Wang; Hong, Zhang; Bing, He; Qun, Li; Zhi-yan, Zou & Jing, Feng

Abstract


Purpose: To investigate the cytotoxic activity of the essential oil of Chenopodium ambrosioides check for this species in other resources L. against human breast cancer MCF-7 cells.
Methods: Cytotoxicity was characterized by 50 % inhibition (IC50) of human breast cancer cell lines (MCF-7) using 3-(4,5-dimethylthaizol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was analysed by Hoechst33258 staining and DNA ladder. MCF-7 cellular superoxide dismutase (SOD), catalase (CAT) vitality and malondialdehyde (MDA) content were evaluated.
Results: The essential oil was cytotoxic to MCF-7 cell line. A dose- and time-dependent inhibition was observed with IC50 values of 18.75, 9.45 and 10.50 μg/ml at 6, 24 and 48 h, respectively. Analyses by Hoechst33258 staining and DNA ladder indicate that the essential oil induced apoptosis. SOD vitality significantly decreased (p < 0.05) by 51 % when the concentration of the essential oil increased from 1.25 to 12.5 μg/ml while CAT vitality significantly increased (p <0.05) by 71 % when essential oil concentration was similarly increased. The MDA content of each treatment group, when compare to control, did not show any significant difference (p < 0.05).
Conclusion: The essential oil of C. ambrosioides was cytotoxic to MCF-7 cell line and induced apoptosis.

Keywords
Chenopodium ambrosioides L.; Essential oil; Cytotoxicity; Apoptosis; Breast cancer; MCF-7 cells

 
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