About Bioline  All Journals  Testimonials  Membership  News

Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 12, No. 6, 2013, pp. 941-948
Bioline Code: pr13119
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 12, No. 6, 2013, pp. 941-948

 en Evaluation of Ginsenoside Rg1 as a Potential Antioxidant for Preventing or Ameliorating Progression of Atherosclerosis
Huang, Gui-dong; Mao, Jian & Ji, Zhongwei


Purpose: To determine whether Rg1 inhibits H2O2-induced injury in human umbilical vein endothelial cells (HUVECs), an injury often regarded as a key early event in the development of atherosclerosis.
Methods: Cell viability of HUVECs treated with Rg1 and/or H2O2 was measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide ( MTT) assay. Lactate dehydrogenase (LDH) release, lipid peroxidation, and reserved oxidase were detected using different available kits. The apoptosis pathway involved in the effect of Rg1 was also evaluated.
Results: Exposing HUVECs to 100 μmol/L H2O2 significantly decreased cell viability (78.12 ± 1.78 %), nitric oxide production, and nitric oxide synthase, superoxide dismutase, and glutathione activities, but markedly increased malondialdehyde content (from 26.87 ± 3.97 to 45.84 ± 3.50 nmol/mg of protein) and LDH release (from 8.63 to 31.42 %) (p < 0.05). These results were accompanied by a decrease in mitochondrial membrane potential and up-regulation of Bid and caspase-3, -8, and -9 mRNA expressions. However, pretreatment with different Rg1 concentrations (4, 8, and 16 µmol/L) markedly attenuated these changes (p < 0.05).
Conclusion: Rg1 may protect HUVECs against H2O2-induced injury via the anti-oxidative and anti-apoptosis mechanisms, which could be applied potentially for the prevention of endothelial cell dysfunctions associated with atherosclerosis.

Ginsenoside Rg1; Human umbilical vein endothelium; Oxidative damage; Atherosclerosis

© Tropical Journal of Pharmaceutical Research
Alternative site location:

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil