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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 1, 2014, pp. 87-92
Bioline Code: pr14013
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 1, 2014, pp. 87-92

 en Antimicrobial, Cytotoxic and Phytotoxic Potency of Ethyl Acetate Extract of Rhizopus stolonifer check for this species in other resources Culture
Iqbal, Mudassar; Amin, Muhammad; Iqbal, Zafar; Bibi, Hamida; Iqbal, Amjad; Din, Zia-ud; Suleman, Muhammad & Shah, Hamid Ullah

Abstract


Purpose: To evaluate the antimicrobial, cytotoxic and phytotoxic activities of the organic extract of Rhizopus stolonifer check for this species in other resources whole cell static culture in order to determine the presence of potentially bioactive compounds in the culture.
Methods: The organic extract was obtained by extracting the whole cell culture of R. stolonifer with Ethyl Acetate (EtOAc). The antifungal activity was determined by inhibitory effect on the growth of Alternaria alternata check for this species in other resources , Aspergillus flavus check for this species in other resources , Curvularia protuberata check for this species in other resources and Fusarium oxysporum check for this species in other resources , cytotoxic activity by brine shrimp lethality test, antibacterial activity against Ervinia carotovora check for this species in other resources and Xanthomonas campestris check for this species in other resources by disc diffusion technique, and phytotoxic activity by testing the crude extract against the Lemna minor frond.
Results: The extract showed phytotoxic activity (at 95% level of significance) against Lemna minor (67.7 % lethality) with Fronds Inhibition (FI50) of 167.85 µg mL-1. The extract exhibited a significant (at 95% level of significance) cytotoxic activity (LC50 of 115.71 µg mL-1) against brine shrimp. Maximum mortality (56.7 %) was obtained at a concentration of 200 µg mL-1 concentration after 48 h. In the antifungal test, the highest inhibitory effect was observed against Fusarium oxysporum (88.8 %) followed by Alternaria alternata (81.5 %), Aspergillus flavus (70.5 %) and Curvularia protuberata (37.5 %) at 1000 µg. mL-1 when compared to negative reference. Antibacterial activity against Ervinia carotovora and Xanthomonas campestris was minimal even at extract level of 2000 µg mL-1.
Conclusion: The ethyl acetate extract of R. stolonifer possesses significant herbicidal, cytotoxic and antifungal properties. Isolation and characterization is required for structural elucidation of its bioactive compounds.

Keywords
Rhizopus stolonifer; Antifungal; Antibacterial; Phytotoxicity; Cytotoxicity

 
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