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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 1, 2014, pp. 149-153
Bioline Code: pr14021
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 1, 2014, pp. 149-153

 en Buffer-Free High Performance Liquid Chromatography Method for the Determination of Theophylline in Pharmaceutical Dosage Forms
Kanakal, Mahibub M.; Majid, Aman Shah Abdul; Sattar, Munavvar Zubaid A.; Ajmi, Shahida Noor & Majid, Amin Malik Shah Abdul

Abstract


Purpose: To develop and validate a simple, economical and reproducible high performance liquid chromatographic (HPLC) method for the determination of theophylline in pharmaceutical dosage forms.
Method: Caffeine was used as the internal standard and reversed phase C-18 column was used to elute the drug and standard. The mobile phase was prepared by mixing water:acetonitrile:methanol at the ratio of 90:03:07 and the pH set at 4.6. Flow rate and ultraviolet (UV) detector were set at 1.0 mLmin-1 and 271 nm, respectively. The method was validated for linearity, recovery, accuracy, precision, specificity, and also for inter-day stability under laboratory conditions.
Results: Retention time was 5.5 min. The limits of detection and quantification were 12.5 ngmL-1 and 100 ngmL-1, respectively. Recovery accuracy (%) for different concentrations ranged from 100.05 to 102.43; regression coefficient (R2) of 0.994; precision RSD < 2.0, and negligible interference from common excipients.
Conclusion: The method is simple, rapid, highly specific and suitable for the determination of theophylline. Absence of buffer and use of small quantity of organic solvents increase the life span of the column and reduce the cost of routine analysis of theophylline in industry.

Keywords
Buffer-free; High performance liquid chromatography; Theophylline; Dosage form

 
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