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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 2, 2014, pp. 225-228
Bioline Code: pr14032
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 2, 2014, pp. 225-228

 en Pectolinarigenin - A Flavonoid Compound from Cirsium Japonicum check for this species in other resources with Potential Anti-proliferation Activity in MCF- 7 Breast Cancer Cell
Lu, Mingqian; Kong, Qingzhi; Xu, Xinhua; Lu, Hongda; Lu, Zhongxin; Yu, Wei; Zuo, Bin; Su, Jin & Guo, Rong

Abstract

Purpose: To isolate high purity pectolinarigenin from Cirsium Japonicum check for this species in other resources and evaluates its antiproliferative potential and induction of apoptosis activity in MCF-7 breast cancer cell.
Methods: Pectolinarigenin was purified by the combination of silica gel and Sephadex LH-20 column chromatography. The structure was identified by 1H-nuclear magnetic resonance (NMR), 13C-NMR and electrospray ionization tandem mass spectrometry (ESI-MS) analyses. The anti-proliferation activity of pectolinarigenin was also evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and colony formation assays in MCF-7 breast cancer cell.
Results: Pectolinarigenin showed potent anti-proliferation activity by inducing apoptosis and downregulation of Bcl2 expression in MCF-7 breast cancer cell. Protein expression of Bcl2 was almost completely inhibited upon the treatment with 25 µM pectolinarigenin.
Conclusion: A rapid method to isolate high purity pectolinarigenin from C. japonicum and as well as evaluate its anti-proliferative potential and induction of apoptosis in MCF-7 breast cancer cells is presented for the first time. Induction of apoptosis of MCF-7 is via downregulation of Bcl2 expression.

Keywords
Cirsium japonicum; Anti-proliferation activity; Pectolinarigenin; Breast cancer; Apoptosis

 
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