Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 13, No. 3, 2014, pp. 417-422
Bioline Code: pr14061
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 13, No. 3, 2014, pp. 417-422
© Copyright 2014 - Tropical Journal of Pharmaceutical Research
Development and Application of Kinetic Spectrophotometric Method for the Determination of Metronidazole|
Miljkovic, Vojkan M.; Kostic, Danijela A.; Bojanic, Zoran Z.; Kalicanin, Biljana M. & Kocic, Gordana M.
Purpose: To develop an improved kinetic-spectrophotometric procedure for the determination of
metronidazole (MNZ) in pharmaceutical formulations.
Methods: The method is based on oxidation reaction of MNZ by hydrogen peroxide in the presence of
Fe(II) ions at pH 4.5 (acetate buffer). The reaction was monitored spectrophotometrically by measuring
the rate of change of absorbance at 317nm.
Results: The optimum operating conditions for reagent concentrations and temperature were
established. Linear calibration curve was obtained in the range of 85.77 – 513.45ng mL-1 with standard
deviation from 1.77 to 4.55 %-. The optimized conditions yielded a theoretical detection limit of 15.20 ng
mL-1 based on 3.3So criterion. Commonly used excipients and other additives such as talc, glucose,
fructose, lactose, starch, magnesium stearate, microcrystalline cellulose and several ions were found
not to have interference.
Conclusion: The developed method is sensitive, accurate and reproducible and could be used for
routine analysis of metronidazole in pharmaceutical preparations.
Metronidazole; Kinetic spectrometry; Validation; Pharmaceutical preparation
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