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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 4, 2014, pp. 573-580
Bioline Code: pr14082
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 4, 2014, pp. 573-580

 en Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from D. radiodurans check for this species in other resources R1 against Carbon Tetrachloride-Induced Liver Injury in Mice
Cheng, Jianhui; Zhang, Zoufa; Zheng, Zhiguo; Lv, Guoying; Wang, Liangyan; Tian, Bing & Hua, Yuejin

Abstract

Purpose: To investigate the antioxidant activity and hepatoprotective effect of deinoxanthin-rich extract from D. radiodurans check for this species in other resources (EDR) against CCl4-induced liver injury in mice.
Methods: The ethanol extract of EDR was analyzed by liquid chromatography/mass spectrometry (LC/MS), and its antioxidant activity was examined using in vitro assays for reducing power, iron chelating activity and lipid peroxidation. The extract was also evaluated for its hepatoprotective activity against carbon tetrachloride-induced liver injury in mice. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) in serum, and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissue, as well as hepatic malondialdehyde (MDA) levels, were measured to monitor liver injury. Damage to liver cells was assessed by histology.
Results: EDR displayed strong reducing activity and lipid peroxidation inhibition activity in vitro. Pretreatment with EDR (400 mg/kg b.w.) significantly reduced activities of serum ALT, AST and ALP, as well as hepatic MDA levels (p < 0.05), but increased the activities of GSH-Px, CAT and SOD. Histopathological assessment showed that liver tissue damage was decreased by the protective effect of EDR.
Conclusion: The results show that EDR can protect mice against CCl4-induced hepatic damage by its antioxidant and free radical scavenging activities.

Keywords
Antioxidant; Deinoxanthin; Deinococcus radiodurans; Hepatoprotective; Carbon tetrachloride; Liver Damage

 
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