To characterize the resistance of Mycobacterium tuberculosis
to second line drugs using a line probe assay.
Multi-drug resistant strains of Mycobacterium tuberculosis
isolated between December 2008 and December 2009 were tested for resistance to fluoroquinolones and second-line injectable drugs using GenoType®
Thirty eight strains gave interpretable results. None of them had a mutation in the gyrA
gene. Regarding second-line injectable drugs, 4 strains (11 %) were resistant to aminoglycosides/ capreomycin and all of them harbored A1401G mutation.
No extensive drug resistant strains were observed. A relatively high proportion of strains were resistant to at least one second-line injectable drug. Resistance mechanism seemed similar for all of them.