To develop and validate an improved method by capillary zone electrophoresis with photodiode array detection for the fingerprint analysis of Ligusticum chuanxiong Hort.
( Rhizoma Chuanxiong
The optimum high performance capillary electrophoresis (HPCE) conditions were 30 mM borax containing 5 %v/v acetonitrile at pH 9.6, direct injection of water extract at 0.5 psi for 5s, 25kV running voltage at positive power, 20 °C capillary temperature and 295 nm detection. Between injections, capillary was rinsed using 0.1M sodium hydroxide solution for 4 min, with HPCE-grade water for 4 min and re-equilibrated with running buffer for 2 min at 20 psi, moreover, the running buffer was replaced after three consecutive injections.
Baseline separation of most of the 17 detectable peaks in the water extract could be completed within 21 min.The relative standard deviations (RSD) of precision, reproducibility, and stability were < 2.79 % for retention time and 8.68 % for peak area of the main peaks.
The developed method is simple, fast, reliable and environment-friendly for the fingerprint analysis of Rhizoma Chuanxiong