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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 5, 2014, pp. 773-778
Bioline Code: pr14110
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 5, 2014, pp. 773-778

 en Development and Validation of Improved Method for Fingerprint Analysis of Rhizoma Chuanxiong check for this species in other resources by Capillary Zone Electrophoresis with Ultraviolet-Diode Array Detection
Wei, Feng Huan; Shen, Qun & Chen, Fei Long

Abstract

Purpose: To develop and validate an improved method by capillary zone electrophoresis with photodiode array detection for the fingerprint analysis of Ligusticum chuanxiong Hort. check for this species in other resources ( Rhizoma Chuanxiong check for this species in other resources ).
Methods: The optimum high performance capillary electrophoresis (HPCE) conditions were 30 mM borax containing 5 %v/v acetonitrile at pH 9.6, direct injection of water extract at 0.5 psi for 5s, 25kV running voltage at positive power, 20 °C capillary temperature and 295 nm detection. Between injections, capillary was rinsed using 0.1M sodium hydroxide solution for 4 min, with HPCE-grade water for 4 min and re-equilibrated with running buffer for 2 min at 20 psi, moreover, the running buffer was replaced after three consecutive injections.
Results: Baseline separation of most of the 17 detectable peaks in the water extract could be completed within 21 min.The relative standard deviations (RSD) of precision, reproducibility, and stability were < 2.79 % for retention time and 8.68 % for peak area of the main peaks.
Conclusion: The developed method is simple, fast, reliable and environment-friendly for the fingerprint analysis of Rhizoma Chuanxiong water extract.

Keywords
Capillary electrophoresis; Rhizoma chuanxiong; Water extract; Fingerprint analysis; Diode array

 
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