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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 5, 2014, pp. 779-786
Bioline Code: pr14111
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 5, 2014, pp. 779-786

 en Optimization and Composition of Volatile Oil from Polygonatum odoratum check for this species in other resources (Mill Druce) using Supercritical Fluid Extraction
Zhao, Xiuhong; Zeng, Jie; Gao, Haiyan & Wang, Ying


Purpose: To determine the effects of temperature, pressure and extraction time on oil yield obtained from Polygonatum odoratum check for this species in other resources as well as the optimum processing conditions for supercritical carbon dioxide (CO2) extraction.
Methods: Supercritical CO2 extraction technology was adopted in this experiment to study the process of extraction of volatile oil from Polygonatum odoratum while gas chromatograph-mass spectrometer (GC-MS) technique was employed to analyze the chemical composition of the volatile oil. Response surface methodology (RSM) was applied to optimize supercritical CO2 process conditions.
Results: The determined optimal conditions were as follows: extraction pressure, 27 MPa; extraction temperature, 50 °C; and extraction time, 97.10 min. Under these conditions, the predicted yield of Polygonatum volatile oil was 2.04 % as against an actual oil yield of 2.02 % of the seeds. GC-MS analysis showed that the extract obtained by supercritical CO2 extraction was richer in fatty acids and flavoring substances than that obtained by hydrodistillation.
Conclusion: Using RSM can optimize conditions of Polygonatum volatile oil. This provides a scientific basis for further research and development of healthy foods, as well as comprehensive utilization of Polygonatum odoratum.

Polygonatum odoratum; Volatile oil; Supercritical fluid; Carbon dioxide; Extraction; Gas chromatograph-mass spectrometer

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