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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 5, 2014, pp. 793-799
Bioline Code: pr14113
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 5, 2014, pp. 793-799

 en High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma
Fagundes, Vinicius HV; Pinho, Rilson J; Wiirzler, Luiz AM; Kimura, Elza; Bersani-Amado, Ciomar A & Cuman, Roberto KN


Purpose: To identify and quantify anethole in the essential oil of fruits of Illicium verum Hook check for this species in other resources (star anise) and in vivo in rat plasma using reverse-phase liquid chromatography.
Methods: Anethole was identified in the essential oil of the fruits of Star anise and determined by gas chromatography-tandem mass spectrometry (GC-MS), nuclear magnetic resonance (NMR), ultraviolet visible spectrophotometry (UV-VIS). A simple, sensitive and validated high performance liguid chromatography (HPLC) technique with UV-VIS detection method was developed for the determination of the compound in rat plasma using: methanol-water (85:15, v/v) as mobile phase at a flow rate of 0.2 ml/min Hypersil ODS Thermo (150 mm × 2.1 mm × 3.0 μM) as column with wavelength detection at 259 nm.
Results: GC determination showed that anethole in the essential oil of star anise exhibited a retention time of 21.02 min. The validation results for anethole in plasma were satisfactory, with coefficient of determination (R2) of 0.9945 and relative standard deviation of < 3 %. HPLC run time of 4 min with a retention time of 2.73 min was the faster method to determine anethole when compared to a previously reported method which had a run time of 15 min.
Conclusion: Anethole in the essential oil of Illicium verum Hook can be identified and determined by GC-MS, NMR and UV-VIS, and a superior HPLC method has been developed for the determination of the compound in rat plasma.

Anethole; High performance liguid chromatography; Star anise; Essential oil; Rat plasma; Illicium verum Hook

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