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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 6, 2014, pp. 863-871
Bioline Code: pr14121
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 6, 2014, pp. 863-871

 en Photoprotective Effect of a Polyopes affinis check for this species in other resources (Harvey) Kawaguchi and Wang (Halymeniaceae)-Derived Ethanol Extract on Human Keratinocytes
Hyun, Yu Jae; Piao, Mei King; Kim, Ki Cheon; Zheng, Jian; Yao, Chen Wen; Cha, Ji Won; Kang, Hee Kyoung; Yoo, Eun Sook; Koh, Young Sang; Lee, Nam Ho; Ko, Mi Hee & Hyun, Jin Won

Abstract


Purpose: To investigate the photoprotective effect of the ethanol extract of the red marine alga, Polyopes affinis check for this species in other resources (PAE) against ultraviolet B (UVB) radiation on cultured human keratinocytes.
Methods: The 2',7'-dichlorodihydrofluorescein diacetate method was used to detect intracellular reactive oxygen species (ROS) generated by H2O2 treatment or UVB radiation. Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ) assay. Superoxide anion or hydroxyl radical was detected using an electron spin resonance spectrometer after reaction with the nitrone spin trap. Lipid peroxidation was assayed by determining the level of 8-isoprostane. Protein carbonyl formation was determined using a protein carbonyl ELISA kit. The degree of oxidative DNA damage was determined using an alkaline comet assay. Apoptosis was assessed by apoptotic bodies and DNA fragmentation.
Results: PAE significantly scavenged the free radical 1,1-diphenyl-2-picrylhydrazyl, as well as hydrogen peroxide- and UVB-induced intracellular ROS. Furthermore, PAE showed 23 % scavenging effect of the superoxide anion and 33 % of the hydroxyl radical. PAE also absorbed UVB rays in the 280 – 320 nm range. PAE significantly decreased cellular damage resulting from UVB-induced oxidative stress to lipids, proteins, and DNA. Furthermore, PAE-treated keratinocytes showed significant reduction in UVB-induced apoptosis, as exemplified by fewer apoptotic bodies and reduced DNA fragmentation.
Conclusion: These results suggest that PAE protects keratinocytes against UVB-induced oxidative stress by absorbing UVB rays and scavenging ROS, thereby reducing injury to cellular constituents.

Keywords
Human keratinocytes; Polyopes affinis; Reactive oxygen species; Red algae; Ultraviolet B; Apoptosis; DNA fragmentation

 
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