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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 6, 2014, pp. 943-949
Bioline Code: pr14132
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 6, 2014, pp. 943-949

 en Elicitation of Valerenic Acid in the Hairy Root Cultures of Valeriana officinalis check for this species in other resources L (Valerianaceae)
Torkamani, Mohammad Reza Dini; Abbaspour, Naser; Jafari, Morad & Samadi, Afsaneh

Abstract


Purpose: To investigate the effect of magnesium and calcium as abiotic elicitors on Valeriana officinalis check for this species in other resources hairy roots for scale-up production of valerenic acid.
Methods: Hairy roots were established in different explants of Valeriana officinalis via a mikimopine type strain of Agrobacterium rhizogenes check for this species in other resources ‘A13’. Transgenic status of hairy roots was confirmed by polymerase chain reaction (PCR) analysis of the rolB gene. Hairy roots of 28-day old cultures were exposed to magnesium and calcium (2 to 6-fold concentration of that present in normal Murashige and Skoog (MS) medium for 3 and 7 days. After elicitation, the cultures were grown in MS liquid medium without elicitors for 8 weeks. Valerenic acid content in hairy roots was analysed using high-performance liquid chromatography (HPLC) to determine the best yield conditions.
Results: Transformed cultures showed significant differences (p < 0.05) in valerenic acid content. The highest amount of valerenic acid (1.83 ± 0.06 mg/g dry weight (DW) was found in hairy root cultures exposed to 6-fold calcium for 7 days, which was 7.9 times higher than that of control culture (0.23 ± 0.01 mg/g DW).
Conclusion: The results show that concentration and exposure time to elicitors are important factors that affect the production of valerenic acid.

Keywords
Valeriana officinalis; Agrobacterium rhizogenes; Elicitors; Explants; Hairy roots; Valerenic acid; Calcium; Magnesium; rolB gene; Transgenic

 
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