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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 7, 2014, pp. 1085-1092
Bioline Code: pr14151
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 7, 2014, pp. 1085-1092

 en Proteomic Analysis of Bacterial Expression Profiles Following Exposure to Organic Solvent Flower Extract of Melastoma candidum check for this species in other resources D Don (Melastomataceae)
Wong, Fai-Chu; Yong, Ann-Li; Sim, Kooi-Mow; Ong, Hean-Chooi & Chai, Tsun- Thai

Abstract

Purpose: To identify potential antibacterial protein targets following exposure to Melastoma candidum check for this species in other resources extract.
Methods: Plant extracts were prepared using sequential extraction method. Denaturing gel electrophoresis and MALDI TOF-TOF MS protein sequencing were used to identify differential- expressed bacterial proteins. 96-well microplate method was used to determine the minimum inhibitory concentration (MIC) values. Thin layer chromatography (TLC) bio-autobiography and gas- chromatography-mass spectrometry (GC-MS) were performed to determine the phytochemicals in the active fraction.
Results: Five differentially expressed bacterial proteins (four from Escherichia coli check for this species in other resources and one from Staphylococcus aureus check for this species in other resources ), were identified via proteomic approach. Among the bacterial proteins identified, glutamate decarboxylase, elongation factor-Tu and α-hemolysin are especially noteworthy, as they are implicated in critical bacterial pathways pertaining to survival in acidic environment, protein translation and virulence, respectively. Additionally, we tested and reported the minimum inhibition concentrations of different M. candidum fractions and gas chromatography-mass spectrometry GC-MS analysis of the active fraction.
Conclusion: Glutamate decarboxylase, elongation factor-Tu and α-hemolysin represent potential antibacterial targets.

Keywords
Escherichia coli; Staphylococcus aureus; Melastoma candidum; Glutamate decarboxylase; Elongation factor-Tu; α-Hemolysin; Protein expression

 
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